Levels of IL-6 in bladder cancer.

A. Levels of IL-6 were examined in six specimens (paired cancer (C) and adjacent non-malignant tissue (N); two specimens in a lane) by RT-PCR and Western blotting analysis. For real-time RT-PCR analysis, the y-axis shows the ratio of IL-6 in cancer tissue divided by that in the non-malignant specimen. Columns, mean of three separate experiments; Bars, standard deviation (SD); *, P<0.05. B. Immunohistochemical staining of human bladder cancer specimens with anti-IL-6 antibody. Representative slides are shown. C. The IHC data showed that IL-6 levels were significantly correlated with clinical stage. D. Urinary IL-6 levels of patients were examined by ELISA analysis. Columns, mean of three separate experiments; Bars, SD; *, P<0.05. (NT, patients with non-malignant diseases).