Lack of effect of Bcl-x<sub>L</sub> over expression on ionomycin-mediated increase in Ca<sup>2+</sup> levels.

<p>a) Time-course of Ca<sup>2+</sup> levels, expressed as percentage of maximum Ca<sup>2+</sup> response, following ionomycin (2 µM; arrow) addition to SH-SY5Y/Neo (solid lines) and SH-SY5Y/Bcl-x<sub>L</sub> cells (dashed lines). Following an initial Ca<sup>2+</sup> peak, a late Ca<sup>2+</sup> increase occurs reaching a plateau that is taken as the maximum Ca<sup>2+</sup> level for the neuron. b) Cytoplasmatic response to ionomycin treatment (2 µM) in SH-SY5Y/Neo (n = 10) and SH-SY5Y/Bcl-x<sub>L</sub> (n = 11) cells measured as the ratio of the early Ca<sup>2+</sup> peak to the maximal ionomycin signal obtained during each individual experiment. Data represent mean ± S.E.M of the indicated number of experiments.</p>