Knockdown of SKN-1 or TEFs, but not TIFs, reduces UPS activity in the intestine.
(A) Representative images of Pvha-6::UbG76V-Dendra2 and Pvha-6::Dendra2 reporters in animals exposed to control, skn-1, eef-1A.1 (TEF) or eif-1(TIF) RNAi. Red fluorescence derives from substrate that was present at the time of photoconversion. Bar: 20 µm. In (A–C), bar graphs depict the percentages of green and red fluorescence compared to either the initial value (t = 0 for green) or point of photoconversion (t = C for red) for UbG76V-Dendra2 and Dendra2, respectively (± SEM). (B,C) Summary of UbG76V-Dendra2 and Dendra2 imaging in intestinal cells after 9 hours in response to TEF and TIF RNAi, respectively. Significant differences relative to control RNAi (red fluorescence after 9 hours) are indicated with **P<0.01, *P<0.05.