Kinetics of FXa and Elastase interactions with rSimukunin.

Responses were obtained by injecting FXa or elastase over immobilized rSimukunin for 180 seconds at a flow rate of 30 µl/minute. Data were derived from Ka1 and Kd1 and fitted using the Langmuir (1∶1 binding) equation. Kinetic values obtained from the sensorgrams presented in Figure 6B and 6C for FXa and elastase respectively.