K562 cell adhesion to RGD containing extracellular matrix proteins is restored in the presence of troglitazone and 9-cis-RA in a concentration and time dependent manner.

(A) K562 cells cultured for 72 hrs in the presence of vehicle (white bars), 1 μM 9-cis-RA (hatched bars), 15 μM troglitazone (gray bars), or 1 μM 9-cis-RA and 15 μM troglitazone (black bars) were added to wells coated with 5 μg/mL of fibronectin, FN-120, or vitronectin. (B) K562 cells cultured for 24, 48, or 72 hrs in the presence of vehicle (white bars), 1 μM 9-cis-RA (hatched bars), 15 μM troglitazone (gray bars), or 1 μM 9-cis-RA and 15 μM troglitazone (black bars) were added to wells coated with 5 μg/mL of FN-120. (C) Microtiter wells were coated with various concentrations of FN-120. K562 cells cultured for 72 hrs in the presence of vehicle (▪), 1 μM 9-cis-RA (•), 15 μM troglitazone (▴), or 1 μM 9-cis-RAand 15 μM troglitazone (○), were added to wells (9×104 cells/well) in Hepes-Tyrodes buffer containing 1 mM MnCl2. Results are expressed as means ± SD, n = 6, LSD was used to test for differences among groups. Means followed by the same letter are not significantly different (P = 0.01).