JNK maintains high levels of GRP78 in human CCA cells.

(A) After treated with SP600125 (SP, 20 µM) for indicated time periods, GRP78 was analyzed using western blot in QBC939, RBE and HCCC-9810 cells. (B) After transfected with siJNK for 60 h, GRP78 was analyzed using western blot in QBC939, RBE and HCCC-9810 cells. (C) After treated with salubrinal (Sal, 25 µM) for 30 h with or without SP600125 (SP, 20 µM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells. (D) After treated with tunicamycin (Tun, 2.0 µg/ml) for 24 h with or without SP600125 (SP, 20 µM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells.