JA responses were not obviously altered in the bhlh3, bhlh13, bhlh14 and bhlh17 single mutants.

(A) Schematic diagrams of T-DNA insertion sites in bHLH3, bHLH13, bHLH14 and bHLH17. White box, UTR; black box, exon; black line, intron; white triangle, T-DNA insertion site. Pair1 and Pair2, indicated by arrows, are the primer pairs for analyzing gene expression in (B). (B) Quantitative real-time PCR analysis of bHLH3, bHLH13, bHLH14 and bHLH17 in the respective T-DNA insertion mutants using primer pairs indicated by arrow pairs in (A). ACTIN8 was used as the internal control. (C) Seedling phenotypes of 7-day-old (upper panel) and 11-day-old (bottom panel) Col-0 wild type (WT), bhlh3, bhlh13, bhlh14 and bhlh17 grown on MS medium supplied without (CK) or with indicated concentrations (µM) of MeJA. (D) Anthocyanin contents of the 11-day-old seedlings in WT, bhlh3, bhlh13, bhlh14 and bhlh17 single mutants grown on MS medium containing indicated concentrations of MeJA. FW, fresh weight. Error bars represent SE (n = 3). (E) Root phenotypes of 7-day-old seedlings of WT and single mutants of bhlh3, bhlh13, bhlh14 and bhlh17 grown on MS medium supplied without (CK) or with indicated concentrations (µM) of MeJA. (F) Root length of 11-day-old seedlings grown on MS medium containing indicated concentrations of MeJA. Error bars represent SE (n = 15). (G) Flowering time of WT, bhlh3, bhlh13, bhlh14 and bhlh17 single mutants. Data shown are the means from 24 plants. Error bars represent SE.