JAM-A expression in NSCLC cells.

JAM-A expression in a panel of human airway-derived cell lines assessed by Western blot, Data are shown as representative Western blots experiments. Experiments were independently repeated 3 times with similar results. (B) Relative expression of JAM-A mRNA in a panel of human airway-derived cell lines assessed by real-time PCR. The relative quantity of JAM-A mRNA, normalized to β-actin, were compared to H157 cells based on the equation RQ = 2^−ΔΔCt. Columns, mean of RQ of triplicate values in a representative experiment; bars, max/min RQ. Experiments were independently repeated 3 times and conducted in triplicate with similar results. (C) Surface expression of JAM-A in a panel of human airway-derived cell lines assessed by a flow cytometry assay. Confluent cells were trypsinized and flow cytometry assay analyses were conducted as described in the Methods section. Data are shown as representative histograms (top panel), mean fluorescence intensity of triplicate values in a representative experiment, Columns, mean; bars,SD. (bottom panel). Experiments were independently repeated 3 times in triplicate with similar results.