Figure_3.tif (149.71 kB)
IscS might participate DNA phosphorothioation directly.
figure
posted on 2012-12-11, 02:09 authored by Xianhui An, Wei Xiong, Yan Yang, Fuhou Li, Xiufen Zhou, Zhijun Wang, Zixin Deng, Jingdan LiangEthidium bromide-stained agarose gels. TAE (top gel), samples run in normal TAE buffer; PAA (bottom gel), samples run in TAE containing PAA. Expression of S. enterica dptB-E resulted in DNA S-modification and a fluorescent smear in all samples, except for E. coli ΔiscS. IscS was therefore the only gene that was required for DNA S-modification among the tested deletions.
