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Involvement of Caspase-1 and classical autophagy pathway in autophagy induced by LPS

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posted on 2013-12-13, 03:04 authored by Shintaro Seto, Keiko Sugaya, Kunio Tsujimura, Toshi Nagata, Toshinobu Horii, Yukio Koide

(A) Autophagy induced by LSP in Caspase-1-KD macrophages. The proportion of macrophages with LC3-dot (upper panel) and immunoblot analysis of LC3 processing (lower panel) are shown. (B) Autophagy induced by LSP in Atg5-KD macrophages. The proportion of macrophages with LC3-dot (upper panel) and immunoblot analysis of LC3 processing (lower panel) are shown. (C) Autophagy induced by LPS in the presence of a PI3K inhibitor, 3-MA. Transfected macrophages were stimulated by LPS in the presence or absence of 3-MA at 10 mM for 24 h. The proportion of macrophages with LC3-dot (upper panel) and immunoblot analysis of LC3 processing (lower panel) are shown. Data represent the mean and SD of three or four independent experiments. *p < 0.05 (unpaired Student’s t-test).

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