Invasive growth of haploid descendants after prolonged nitrogen limitation.

Descendants of CEN.PK113-7D in A, glutamine-limited culture, and C, ammonium-limited culture, grown on rich complex medium (YPD) for 3 days at 30°C and flushed with water to determine the proportion of adhering clones (B and D). Of descendants from the glutamine-limited population, 80% were invasive on YPD, while in the two ammonium-limited populations, 40% and 26% percent had become invasive (n>200).