Intracellular synthesis of pro- and anti-inflammatory cytokines in native CD14+ monocytes.

Native CD14+ cells (n = 4) were incubated with LPS 100ng/ml, 100μg/ml Curosurf, 100μg/ml POPG, 100μg/ml PLs, 100μg/ml PLs+SP-B, 100μg/ml PLs+SP-C and 100μg/ml CHF5633 for 14h. Unstimulated CD14+ cells served as negative control, LPS-stimulated monocytes served as positive control. Intracellular concentrations of TNF-α (A), IL-1β (B), IL-8 (C) and IL-10 protein (D) are given as mean ± SD (*p < 0.05, **p < 0.01).