Innate immunity features from cynomolgus macaques naturally infected with T. cruzi (CH) and non-infected controls (NI).
(A) Flow cytometry immunophenotyping platforms were assembled to quantify the percentage of macrophage-like (CD14+CD16+) and pro-inflammatory (CD14+CD16+HLA-DR++) events within gated monocytes. Activation status was estimated by the analysis of CD56 and FcγR (CD32, CD64) expression by circulating monocytes, and data are reported as the Mean Fluorescence Intensity (MFI). (B) Analyses of NK-cells were performed to quantify NK subpopulations (CD3-CD16+CD56-, CD3-CD16+CD56+ and CD3-CD16-CD56+), the cytotoxicity profile (Granzyme A, Granzyme B and Perforin) and activation markers (CD69 and CD54). The results are expressed as mean percentage with standard error. Significant differences at p<0.05 are identified by asterisks (*).