Inhibition of the Th2 response with anti-IL-4 antibodies does not confer anakinra or Dex resistance in IL-17A production by antigen-restimulated lung cells in NO2-promoted allergic airway disease.
Mice were treated as in Figure 2. Lung single cell suspensions were restimulated with OVA antigen in the presence or absence of anakinra (Ana; 1 μg/mL), Dex (10-8 M), or anakinra and Dex in combination for 96 hours. Cell supernatants from antigen-restimulated and treated lung cells from IgG isotype control treated mice (A-D) or anti-IL-4 treated mice (E-H) were analyzed for cytokines by ELISA. Statistics were performed by one-way ANOVA and Bonferroni post hoc analysis (E-L). **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05 compared to controls (none) unless otherwise indicated by brackets. ND, not significantly different compared to controls. n = 6-12 per group.