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Inhibition of Mapk3/1 activity and knockdown of Egr1 results in reduced induction of Ptgs2 in primary granulosa cells in vitro.

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posted on 2015-03-24, 04:57 authored by Dayananda Siddappa, Élaine Beaulieu, Nicolas Gévry, Philippe P. Roux, Vilceu Bordignon, Raj Duggavathi

A) Treatment with PD0325901 decreased Fo+PMA induced increases in the abundance of Ptgs2 protein relative to vehicle treated primary granulosa cells. B) Pre-treatment with Egr1-siRNA inhibited Fo+PMA induced expression of Egr1 when compared to control siRNA treatment in primary granulosa cells in vitro. This was associated with reduced abundance of Pgts2 transcript in Egr1-siRNA treated cells. Fo—forskolin; PMA—phorbol-12-myristate.

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