In vivo activity of UGT73C14 against ABA.
(A) Relative transcript abundance of G. hirsutum UGT73C14 in transgenic Arabidopsis lines evaluated by RT-qPCR analysis. Error bars represent the standard deviation from three plants of line. (B) Transgenic Arabidopsis thaliana seeds overexpressing UGT73C14 (lines 9, 17, and 23) and wild type (Wt) were plated on phytagel supplemented with 2.2g/L Murashige and Skoog basal salts at pH 6.0. Each transgenic or wild type line was plated with and without filter sterilized 0.5μM Abscisic Acid in the phytagel. After 4 days at 4°C in darkness, germination occurred over 14 days in 8: 16 hr dark: light cycles at 18°C and 22°C, respectively. The percentage of germinated seedlings showing 2 emerged cotyledons was determined on days 0, 3, 7, 10 and 14. Statistical analysis of cotyledon emergence rate between transgenic and wild type plants is provided in Data S1. Error bars represent the standard deviation. (C) Representative picture of cotyledon emergence of wild type and UGT73C14 in the absence (left) or presence of 0.5 µM Abscisic Acid (right). The pictures were taken on day 6 after planting of T2 transgenic seeds of line 9 and wild type.