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Immunofluorescence analysis of muscle basement membrane and plasma membrane.

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posted on 05.05.2015 by Nobuaki Chinzei, Shinya Hayashi, Takeshi Ueha, Takaaki Fujishiro, Noriyuki Kanzaki, Shingo Hashimoto, Shuhei Sakata, Shinsuke Kihara, Masahiko Haneda, Yoshitada Sakai, Ryosuke Kuroda, Masahiro Kurosaka

The expression of DAPI, laminin, and dystrophin was examined in wild-type (WT; upper panel) and p21 knockout (KO; lower panel) mice. (A) Expression in control, (B) Expression in 3 days, (C) Expression in 14 days, and (D) Expression in 28 days post-operation. (Scale bar = 50μm). (E) Quantification of laminin expression, (F) Quantification of dystrophin expression. (A-F) The injured membrane structure was almost repaired 14 days after injury in WT group, but not in the p21 KO group (p < 0.05).

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