Immunofluorescence analysis of muscle basement membrane and plasma membrane.
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The expression of DAPI, laminin, and dystrophin was examined in wild-type (WT; upper panel) and p21 knockout (KO; lower panel) mice. (A) Expression in control, (B) Expression in 3 days, (C) Expression in 14 days, and (D) Expression in 28 days post-operation. (Scale bar = 50μm). (E) Quantification of laminin expression, (F) Quantification of dystrophin expression. (A-F) The injured membrane structure was almost repaired 14 days after injury in WT group, but not in the p21 KO group (p < 0.05).