Immunofluorescence (IFC) and immunocytochemistry (ICC) results for evaluation of CSFV proliferation amount.
A) IFC result of CSFV proliferation amount in the four cell lines p.i. The horizontal panels are different cell lines with CSFV challenge while the vertical ones represent different time points post CSFV challenge. The white spots denote CSFV virions, the brighter the spot is the more CSFV virions the cell contains. B) ICC result of CSFV proliferation amount in different cell lines p.i. The same with Fig. 2A, the horizontal and vertical panels denote different cell lines and different time points post CSFV challenge, respectively. The brown spots denote CSFV virions. The deeper the color is, the more amount of CSFV virions the cell contains. The exposure time for IFC and the background for ICC were kept consistent within each set of expreiments.