Immunoblotting analysis of pSTAT3, and evaluation of IL-6 secretion after DHA treatment.
A: Human visceral adipocytes, collected from the four groups of subjects, were serum-starved for 18 h. Whole cell extracts were separated by SDS-PAGE and analyzed using anti-pSTAT3 antibody. Results were normalized to STAT3 protein content. The data are expressed as means ± SEM. Representative blots are shown. B: IL-6 release was evaluated in the culture media by Elisa as described in Materials and Methods.
Data are expressed as means ± SEM. *, P<0.05 with respect to the untreated paired cells. NW: normal weight subjects (n=5); Ob: overweight/obese subjects (n=5); NWCC: normal weight with colorectal cancer (n=7); ObCC: overweight/obese with colorectal cancer (n=7).