IgG reaction pattern associated to DTH conversion.
Total protein extract from L. chagasi was SDS-PAGE resolved (10% polyacrilamide) and electrotransferred to nitrocellulose filter. In all blots, sera were used at 1∶1000, and secondary anti-human IgG was used at 1∶2000. A) Sera from six VL patients before and after DTH+ conversion. B) Western blot results using sera from active VL and recovered VL patients, asymptomatic infected and non-infected individuals. Molecular weight markers are shown on the left panel.