Figure_1.tif (882.77 kB)
IgG reaction pattern associated to DTH conversion.
figure
posted on 2012-06-19, 00:19 authored by Daniel R. Abánades, Leonardo V. Arruda, Elaine S. Arruda, José Roberto A. S. Pinto, Mario S. Palma, Dorlene Aquino, Arlene J. Caldas, Manuel Soto, Aldina Barral, Manoel Barral-NettoTotal protein extract from L. chagasi was SDS-PAGE resolved (10% polyacrilamide) and electrotransferred to nitrocellulose filter. In all blots, sera were used at 1∶1000, and secondary anti-human IgG was used at 1∶2000. A) Sera from six VL patients before and after DTH+ conversion. B) Western blot results using sera from active VL and recovered VL patients, asymptomatic infected and non-infected individuals. Molecular weight markers are shown on the left panel.
