Identification of proteins forming SDS-insoluble aggregates in cells expressing 103Q-GFP.
2D-DIGE image of proteins of aggregates isolated using SDS (A). Spots corresponding to proteins from cells expressing 103Q-GFP and 25Q-GFP (control) are red and green, respectively. SDS-PAGE image of proteins from the same DRAFs that were insoluble in UTC buffer (B). Mr – protein molecular mass markers. Proteins identified by mass-spectrometry are indicated. Identification data are presented in Table 5 and S5 Table.