Identification of histones H3 and H4 in sperm nuclei of E. sinensis.

(A) Sperm nuclear proteins separated by 15% SDS-PAGE. Individual arrows show histones H3 and H4 respectively. (B) Western blot of sperm nuclear proteins labeled with an anti-histone H3 antibody. (C) Western blot of sperm nuclear proteins labeled with an anti-histone H4 antibody. Key: M, marker protein; SP, sperm nuclear proteins.