IFNβ reduces mutant p53 mRNA levels by inhibiting its RNA stabiliser WIG1 (ZMAT3).
Cells were treated with IFNβ for the designated time points, fixed and sorted by an “Image stream” FACS. (A) Representative images from each condition and (B) a graph representing the mean pixel intensity of mutant p53 positive cells for the entire population. (C) Cells were treated with the designated IFNs for 24 h and mutant p53 and GAPDH levels were measured by western blot. Cells were treated with IFNβ for 24 h and mutant p53 (D) and WIG1 (E) RNA levels were determined by QRT-PCR. * P<0.05. (F) HepG2 cells were introduced with mut175 plasmid and treated with IFNβ. RNA levels were determined by QRT-PCR. * P<0.05. (G) H1299 cell harbouring a TS form of mutant p53 (Wild type form at 32°C and mutant form at 37°C) were treated with IFNβ for 30 h. p53 and GAPDH levels were measured by western blot, shown in the lower panel a is normalized quantification of the bands.