ICP0 co-localizes with reorganized microtubules in HSV-1 infected cells.

Immunofluorescent staining of Vero cells that were uninfected (no virus) or that were inoculated with 5 pfu per cell of HSV-1 0ΔRING, HSV-1 0+GFP105, or wild-type HSV-1 strain KOS. Cells were inoculated in the presence of 200 µM cycloheximide from −0.5 to 10 hours p.i., and were released into medium containing no drugs. At 4 hours post-release, uninfected cells and KOS-infected cells were fixed and stained with antibodies against α-tubulin (rabbit IgG, Alexa Fluor 594) and ICP0 (mouse IgG, fluorescein). Nuclei were counterstained with the DNA-binding dye Hoechst 33342. HSV-1 0ΔRING and 0+GFP105-infected cells were fixed and stained for α-tubulin, and the ICP0ΔRING and ICP0+GFP-105 proteins were visualized using their GFP fluorophores. The scale bar denotes a distance of 10 µm.