Homodimerization of SULT4A1.
(A) Conservation of the dimerization motif in various human SULTs. The consensus sequence is shown below. (B) Immunoprecipitaton assay for dimerization of SULT4A1 protein. IMR-32 cells were co-transfected with HA-tagged SULT4A1 and either FLAG-tagged wild-type SULT4A1 (WT), mutant SULT4A1 (TV) or empty vector (EV). Isolated cytosolic proteins were immunoprecipitated with anti-HA antibody and detected with anti-FLAG antibody. Western blots of protein lysates prior to immunoprecipitation (Input) demonstrate expression. Western blots using anti-HA antibody (lower panel) demonstrates immunoprecipitation.