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High-resolution SEM analysis of tip links.

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posted on 2013-02-19, 18:33 authored by Kateri J. Spinelli, Peter G. Gillespie

(a, b) Control hair bundles had moderate levels of bundle Fluo-4 fluorescence and many tip links at their stereocilia tips (c, d; orange arrowheads point to tip links). (e, f) Bundles treated with 5 mM EGTA for 5 min showed a marked decrease in bundle Fluo-4 fluorescence and a significant reduction in the number of tip links (g, h). Red stars in e and f highlight cell body Fluo-4 fluorescence. (i) Tip links were quantified by dividing the number of tip links in a bundle by the number of observable tip link positions (**** p<0.0001). Scale bars in a and e are 10 µm, c and g are 1 µm, and d and h are 0.5 µm.

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