High Neto levels affect the BMP signaling.
(A) Confocal images of NMJ4 (segment A4) in larvae of indicated genotypes labeled for GluRIIA (green), pMad (red), and HRP (blue). Excess Neto induced a decrease in the intensity but not number of synaptic GluRIIA and pMad signals (quantified in B-C). All samples were processed and imaged similarly using at least ten NMJs per genotype. Error bars indicate SEM. *; p<0.001, **; p<0.01, ***; p<0.05. Bars: 10 μm.