Figure_5.tif (708.34 kB)

HDAC and mTORC1 inhibition in Myc-CaP cell lines in vitro result in alterations of androgen receptor and HIF-1α protein levels with loss of transcriptional activity.

Download (0 kB)
figure
posted on 07.11.2011 by Leigh Ellis, Kristin Lehet, Swathi Ramakrishnan, Remi Adelaiye, Kiersten M. Miles, Dan Wang, Song Liu, Peter Atadja, Michael A. Carducci, Roberto Pili

(A) Myc-CaP cell lines with stable expression of androgen response element [Myc-ARE] plasmid treated with 10 nM R1881±10 nM panobinostat [P], 10 nM everolimus [E] or combination. At indicated times cells were lysed and luminescence intensity was measured and quantitated. Mean ± SE from 3 independent experiments. (B) AR and human c-Myc protein was investigated by immunoblot using whole cell Myc-CaP lysates after treatment with 10 nM R1881 with or without 10 nM panobinostat, 10 nM everolimus or combination for 24 hours. β-actin was used as loading control. (C) Myc-CaP cell lines with stable expression of hypoxia response element [Myc-HRE] plasmid treated with 100 µM CoCl2±10 nM panobinostat [P], 10 nM everolimus [E] or combination. At indicated times cells were lysed and luminescence intensity was measured and quantitated. Mean ± SE from 3 independent experiments. (D) HIF-1α protein was investigated by immunoblot using whole cell Myc-CaP lysates treated with 100 µM CoCl2 with or without 10 nM panobinostat, 10 nM everolimus or combination for 24 hours. β-actin was used as loading control. * p<0.05.

History

Licence

Exports