Gentiolactone does not inhibit IκB degradation induced by LPS stimulation.
RAW264.7 cells were cultured with LPS (100 ng/mL) and gentiolactone (500 µM) for the indicated periods. Cell extracts from each group were examined by immunoblotting analysis using anti-IκBα antibody. Plotted values represent the mean value ± SD from four independent cultures. Helenalin was used as a positive control.