Figure_1.tif (132.63 kB)
Genetic layout of the rVSV-EnvG4-G6 vector, HIV-1 Env, and the EnvG insert.
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posted on 2014-09-12, 02:51 authored by Svetlana Rabinovich, Rebecca L. R. Powell, Ross W. B. Lindsay, Maoli Yuan, Alexei Carpov, Aaron Wilson, Mary Lopez, John W. Coleman, Denise Wagner, Palka Sharma, Marina Kemelman, Kevin J. Wright, John P. Seabrook, Heather Arendt, Jennifer Martinez, Joanne DeStefano, Maria J. Chiuchiolo, Christopher L. ParksVSV genes are shown in the 3′ to 5′ orientation as ordered on the recombinant genomic VSV plasmid and are not to scale. Arrows below each VSV gene depict the diminishing 3′-to-5′ mRNA transcription gradient. ss: signal sequence. MPER: membrane proximal external region. TM: transmembrane domain. CT: cytoplasmic tail domain. Star denotes site of intracellular Env(G) cleavage by furin.
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G positionSignificant quantitiesimmunogenic HIVConformationally Intacthumoral responsesnovel vectorcytoplasmic tail regionssurface glycoproteinG generegimen elicitingFunctional HIVflow cytometryEnvG functionalityIM VSVsurface EnvGEnv trimersHIV vaccine platformclade B Env immunogenattenuating quantitiesSIV challengestomatitis virusgrowth attenuationelisaattenuated HIVHIV immunogensElicits Potent CellularEnvelope TrimersWestern Blot analysissurface Gilrhesus macaquesneutralizing Ab titersIM electroporated plasmids encoding EnvGsafety concerns
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