Genes and the related biological processes in mouse liver regulated by fasting and refeeding.

(A, C) The 8815 selected genes as described in Materials and Methods and Figure S3A were separated into nine distinct clusters according to the genes upregulated, downregulated or unaffected by fasting and refeeding compared with normal feeding. Cluster 1 included 83 genes upregulated by fasting and refeeding. Cluster 2 included 50 genes upregulated by fasting and downregulated by refeeding. Cluster 3 included 339 genes upregulated by fasting and unaffected by refeeding. Cluster 4 included 128 genes downregulated by fasting and upregulated by refeeding. Cluster 5 included 123 genes downregulated by fasting and refeeding. Cluster 6 included 786 genes downregulated by fasting and unaffected by refeeding. Cluster 7 included 618 genes unaffected by fasting and upregulated by refeeding. Cluster 8 included 178 genes unaffected by fasting and downregulated by refeeding. Cluster 9 included 6510 genes unaffected by fasting and refeeding. N, gene abundance under normal feeding condition; F, gene abundance under fasting condition; R, gene abundance under refeeding condition. (B, D) The clustered genes were assigned to different biological processes based on Gene Ontology using the web tool DAVID. The top biological processes and the case genes in each cluster ranked by P-value were listed (P<0.001, case genes ≥5).