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Generation of IEC-specific Shp2 CKO mice.

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posted on 2014-03-27, 03:51 authored by Hironori Yamashita, Takenori Kotani, Jung-ha Park, Yoji Murata, Hideki Okazawa, Hiroshi Ohnishi, Yonson Ku, Takashi Matozaki

A: Genomic DNA extracted from the indicated organs of adult control (Ctrl) or Shp2 CKO (CKO) mice was subjected to PCR analysis with primers specific for deleted or floxed alleles of Ptpn11. Data are representative of three separate experiments. B: Lysates of IECs (from the ileum or colon) and the indicated organs from control or Shp2 CKO mice were subjected to immunoblot analysis with antibodies to (α-) both Shp2 and β-tubulin (loading control). Data are representative of three (IECs) or two (indicated organs) separate experiments.

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