GS2 KD in moody-positive subperineurial glia reveals selective labeling of perisynaptic glial cells.

Confocal immunofluorescence images of DLM neuromuscular synapses as described in Fig 1. In contrast to the distribution of GS2 observed in wild-type (A, B), GS2 was restricted to perisynaptic glia following Gs2-RNAi KD using the moody-GAL4 driver (C,D). (E-G) The spatial distribution of perisynaptic glial cells over the muscle surface indicates that each occupies a distinct territory in which it makes contacts with local synapses.