Figure_3.tif (1.78 MB)

GRIM-1 regulates small non-coding RNA levels.

figure

posted on 2011-09-08, 02:25 authored by Shreeram C. Nallar, Limei Lin, Varsha Srivastava, Padmaja Gade, Edward R. Hofmann, Hafiz Ahmed, Sekhar P. Reddy, Dhananjaya V. Kalvakolanu

Relative abundance was calculated with respect to spliceosomal RNA (snRNA-U6) levels by real-time PCR. A) Levels of the indicated RNA in HeLa cells stably expressing Myc-tagged GRIM-1 isoforms. GRIM-1 isoforms differentially suppress box H/ACA-containing RNA. Top: snoRNA-73 (open), -74 (hatched), -50 (light gray); Middle: scaRNA-8 (stippled), -13 (brick), TERC (black); Bottom: spliceosomal RNA (snRNA-U2 (wavy)) and box C/D-containing RNA (snoRNA-U3 (checkered)) levels. A related box C/D snoRNA-U13 (dark gray) levels are not influenced by GRIM-1. B) Levels of the indicated RNA upon GRIM-1 depletion in HeLa cells. GRIM-1-depleted cells (GS) show increased box H/ACA-containing RNA. Top: snoRNA-73, -74 and -50; Middle: scaRNA-8, -13, TERC; Bottom: snRNA-U2 and snoRNA-U3 levels. A related box C/D snoRNA-U13 levels is not influenced by GRIM-1. Bar patterns same as in (A). **, p<0.01.