GM-CSF increased Mcl-1 levels in standard purity and highly purified neutrophils between 8 and 20 hours.

A. Rates of apoptosis (top) and Mcl-1 expression ± GM-CSF as determined by western blot analysis in neutrophils isolated using the Percoll method. n = 3 independent experiments from different donors. *P<0.05, *** p<0.001, one-way ANOVA, with Bonferroni's post-test correction for multiple comparisons. B. Rates of apoptosis (top) and Mcl-1 expression ± GM-CSF as determined by western blot analysis in neutrophils isolated using the OptiPrep (+NMS) method. n = 3 independent experiments from different donors. *P<0.05 one-way ANOVA, with Bonferroni's post-test correction for multiple comparisons.