Functional sites and their conservation in Tat.

Output of HIVToolbox2 for Tat. A. Surface plot of Tat (1TAC) with functional site amino acids colored. Colors are ADP ribosylation sites (blue), proteolysis site (cyan), dimerization site (purple), phosphorylation sites (dark brown, teal), acetylation sites (tan, orange), RNA binding site (brown), methylation sites (red, royal blue), ubiquitination site (gray), and cell attachment site (green). Other sites on the opposite face are not shown. B. Surface Plot showing residues >90% conserved in 2482 Tat sequences (yellow) C. Protein Sequence of Tat. Highlighted colors are as described in A. Mapping of functional site (highlighted fonts) and protein-protein interaction sites (lines underneath sequence). These lines map Tat interaction with Cyclin T1, CDK9, CDK2, Lysine acetyl transferase 2B, 5, Tat interaction protein, Transcription elongation factor 1, p53, p73, Zinc finger and BTB domain containing 7A, Early growth response 1, BCL2-like 11, Protein phosphatase 1, Tubulin α4a, TBP-associated factor 1, several PKCs, and PKD3, Histone cluster 1, Karyopherin β1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2, DNA directed RNA polymerase II, Eukaryotic translation initiation factor 2α kinase 2 (left to right). The blue shaded box shows residues 15–57.