Frequency of spreading of sequence from a single tagged telomere to other telomeres in <i>K. lactis stn1-M1</i> mutants with different genetic backgrounds.

2012-11-01T02:45:18Z (GMT) by Jianing Xu Michael J. McEachern
<p>Columns 1–8 show histograms of the frequencies of four different types of outcomes (none, slight, intermediate and complete/near complete) with regard to the extent and spreading of ApaL repeats in newly generated <i>stn1-M1</i> mutants. Numbers above each column indicate the total number of clones examined. Numbers within histograms indicate the number of clones of each type when those numbers were above zero. Definitions of the outcome types are as follows. None: defined by each of the following being true: 1) total <i>Eco</i>RI-digested telomeric signal is reduced no more than 1.5 fold by additional <i>Bcl</i>I digestion; 2) Bcl specific telomeric signal is less than 2.5 fold more than that of “precursor”; 3) <i>Eco</i>RI-digested subtelomeric signal larger than 2 kb is reduced no more than 1.5 fold by <i>Bcl</i>I digestion. Slight: defined by each of the following being true: 1) total <i>Eco</i>RI-digested telomeric signal is reduced no more than 3 fold by additional <i>Bcl</i>I digestion; 2) Bcl specific telomeric signal is less than 5 fold more than that of “precursor”; 3) <i>Eco</i>RI-digested subtelomeric signal larger than 2 kb is reduced more than 1.5 fold by <i>Bcl</i>I digestion. Intermediate: defined by each of the following being true: 1) total <i>Eco</i>RI-digested telomeric signal is reduced no more than 3 fold by additional <i>Bcl</i>I digestion; 2) Bcl specific telomeric signal is 5 fold or more higher than that of “precursor”; 3) <i>Eco</i>RI-digested subtelomeric signal larger than 2 kb is reduced more than 1.5 fold by <i>Bcl</i>I digestion. Complete/Near Complete: defined by each of the following being true: 1) total <i>Eco</i>RI- digested telomeric signal is reduced at least 3 fold by additional <i>Bcl</i>I digestion; 2) Bcl specific telomeric signal is 8 fold or more than that of “precursors”; 3) <i>Eco</i>RI-digested subtelomeric signal larger than 2 kb is reduced more than 1.5 fold by <i>Bcl</i>I digestion.</p>