Frequency of JR-FL Env-specific CD4+ T cell responses in the lung and spleen.

1.5×106 leukocytes isolated from the lungs (a, b) andspleen (c, d) at the end of the study were stimulated ex vivo with JR-FL gp140, anti-CD28 and brefeldin A before being analyzed by flow cytometry for production of IFNg, IL-2 and TNFa. The total frequency of cytokine secreting CD4+ T cells (%) producing IFNγ, IL-2 or TNFα are shown on the left (a, c) and the frequency of multifunctional CD4+ T cells producing any combination of IFN γ +, IL-2+, and/or TNFα+ on the right (b, d). *p<0.05 compared to group 1. #p<0.05 compared to group 2. ∧p<0.05 compared to group 4. p<0.05 compared to group 5. @p<0.05 compared to group 6. xp<0.05 compared to group 7. Bars represent the median (with SEM), individual animals are shown.