Flux to pyrimidine nucleotide synthesis induced by HSV-1 infection.
(A) Schematic of carbon labeling from glutamine to UTP arising during carbon influx from glutamine to the TCA cycle. Red dots denote 13C atoms originating from uniformly 13C-labeled glutamine. (B) Plots show the levels of the labeled form of the indicated metabolites expressed as percent of the total metabolite pool. The labeling arose upon switching HSV-KOS infected or mock treated HFF cells to uniformly 13C-labeled glutamine media. HCMV infected cells were switched at 48 hpi, HSV-1-infected cells at 12 hpi. The x-axis indicates time post media switch (mean ±2 s.e.; n = 2).