Flowering time of SUC2prom::MYB30 and myb30 mutant plants in LD and SD conditions.
(a)WT and SUC2prom::MYB30 expressing Col plants were grown in LDs (16 h light) and SDs (8 h light) in a temperature controlled climate chamber. The number of rosette and cauline leaves was counted to determine flowering time. Pictures were taken after the WT plants had started to bolt in each condition. Statistical significance was determined using the Student’s t-test (p<0.01). Significant differences are indicated by different letters above the bars, SD and LD treatments are tested as separate experiments. (b) Position of T-DNA insertion lines of myb30. A 400 bp PCR fragment used to detect expression is indicated. (c) Semi-quantitative RT-PCR confirms myb30 mutants as loss-of function alleles. (d) Flowering time of Col, myb30_1, myb30_2 and SUC2prom::MYB30 plants were measured in LD (left) and SD (right) conditions in a temperature controlled climate chamber. Statistical significance was determined using the Student’s t-test by comparing each genotype to the respective Col control (p<0.01). Significant differences are indicated by different letters above the bars. The number of plants for each genotype (n) is indicated below the graph.