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Flow cytometry analysis of cell death in CD14⁺ monocytes after exposure of cells to LPS and surfactant preparations.

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posted on 2016-01-28, 12:38 authored by Kirsten Glaser, Markus Fehrholz, Tore Curstedt, Steffen Kunzmann, Christian P. Speer

Viability of cells was assessed by flow cytometry staining using APC-H7-conjugated viability dye labeling dead cells prior to staining for intracellular antigens. Dead cells are displayed in upper left and upper right quadrant.

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generation reconstituted lps CHF 5633 effects 4 h surfactant CHF 5633 TLR 2 TLR 4 mRNA CHF 5633 pcr flow cytometry.ResultsNeither CHF 5633 Reconstituted Surfactant CHF 5633 intracellular cytokine synthesis monocyte tnf il Protein expression

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Flow cytometry analysis of cell death in CD14+ monocytes after exposure of cells to LPS and surfactant preparations.