Figure 1

A) MM6 cells: non-infected or MM6 cells infected at an m.o.i. of five with the indicated lentiviral vector were cultured for five days. Cells were then treated with LPS for 24 hours as indicated. Cell extracts were prepared and immunoblots performed to measure levels of Cyclin T1, CDK9, and β-actin. B) Jurkat cells: non-infected or Jurkat cells infected at an m.o.i. of five with the indicated lentiviral vector were cultured for five days. Cells were then treated with PMA+ ionomycin for 24 hours as indicated. Cell extracts were prepared to measure expression of Cyclin T1, CDK9, and β-actin.