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Fibroblastoid colony forming unit (CFU-f) frequency and immunophenotype.

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posted on 2012-05-25, 00:56 authored by Michael Lohmann, Gudrun Walenda, Hatim Hemeda, Sylvia Joussen, Wolf Drescher, Stefan Jockenhoevel, Gabriele Hutschenreuter, Martin Zenke, Wolfgang Wagner

Mononuclear cells were isolated from bone marrow and seeded in dilution series with media containing 31 different HPLs or FCS – these experiments were performed with three individual MSC preparations. CFU-f frequency was assessed after 7 days via crystal violet staining (A). CFU-f frequency varied between different MSC preparations, but there was no association with HPL-donor age within either of the MSC preparations (B). Subsequently, cell preparations were expanded for one additional passage. Flowcytometry revealed the commonly described surface marker profile for MSC (C; autofluorescence =  black line). HPLs derived from younger donors (<35 years; n = 5), or HPLs derived from older donors (>45 years; n = 5) did not have significant impact on the immunophenotype (D). *P<0.05; scale bar = 500 µm.

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