Fall in circulating platelets induced by the i.v. injection of collagen and phenylephrine.

Blood was collected from the yugular vein of isofluorane-anesthetized mice followed by i.v. injection of 150 ng collagen type I plus 15 ng of epinephrine per g of mice body weight, as described in Methods. At the indicated time blood was withdrawn from the contralateral yugular vein and the number of platelets determined. Seven animals were analyzed in each group. The values are means ±SEM. By t-test * p<0.05.