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Expression stability of 12 candidate reference genes analyzed using geNorm for qRT-PCR in Yesso scallop tissues (A, B) and embryos/larvae (C, D).

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posted on 2013-09-19, 02:16 authored by Liying Feng, Qian Yu, Xue Li, Xianhui Ning, Jing Wang, Jiajun Zou, Lingling Zhang, Shi Wang, Jingjie Hu, Xiaoli Hu, Zhenmin Bao

The expression stability (M) of each gene was evaluated during stepwise exclusion of the least stable gene (A, C). The optimal number of reference genes required for accurate normalization was determined through calculating pairwise variation Vn/Vn+1(B, D).

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