Figure_3.tif (2.23 MB)

Expression patterns of inflammation, cellular proliferation and angiogenesis genes after UV treatment.

figure

posted on 2014-06-11, 03:04 authored by Deepika Malik, Tiffany Hsu, Payam Falatoonzadeh, Javier Cáceres-del-Carpio, Mohamed Tarek, Marilyn Chwa, Shari R. Atilano, Claudio Ramirez, Anthony B. Nesburn, David S. Boyer, Baruch D. Kuppermann, S. Michal Jazwinski, Michael V. Miceli, Douglas C. Wallace, Nitin Udar, M. Cristina Kenney

The 0-hour values for H-untreated cybrids were normalized to 1 and then compared to gene expression values at 0, 72 and 120 hours. Panel 3a: At 0-hour, the J-untreated cybrids (black solid line) had lower expression levels of IL-33 compared to H-untreated cybrids (grey solid line, p<0.0001). At 120 hours, the H-untreated cybrids (grey solid line) showed higher levels of IL-33 expression compared to the J-untreated cybrids (black solid line). At both 72 and 120 hours, the J-treated cybrids showed higher levels of IL-33 expression compared to the H-treated cybrids. Panel 3b: At 0-hours, the TGF-A expression levels were lower for the J-untreated cybrids (black solid line) compared to the H-untreated cybrids (grey solid line, p<0.0001). At 120 hours after UV-treatment, the TGF-A levels for both H-treated (grey broken line) and J-treated (black broken line) were higher than the untreated H (grey solid line) or J (black solid line) cybrids. Panel 3c: At 0-hours, the expression levels for EFEMP1 were lower in the J-untreated (black solid lines) compared to H-untreated cybrids (grey solid lines, p = 0.0001). By 120 hours, the J-treated cybrids (black broken line) and J-untreated cybrids (black solid line) showed greater fold increases of EFEMP1 expression compared to the H-treated (grey broken line) and H-untreated (grey solid line) cybrids. There were triplicate wells for each sample and the experiments were repeated twice. J cybrids, n = 3 different individuals; H cybrids, n = 3 different individuals.