Expression patterns of inflammation, cellular proliferation and angiogenesis genes after UV treatment.

The 0-hour values for H-untreated cybrids were normalized to 1 and then compared to gene expression values at 0, 72 and 120 hours. Panel 3a: At 0-hour, the J-untreated cybrids (black solid line) had lower expression levels of IL-33 compared to H-untreated cybrids (grey solid line, p<0.0001). At 120 hours, the H-untreated cybrids (grey solid line) showed higher levels of IL-33 expression compared to the J-untreated cybrids (black solid line). At both 72 and 120 hours, the J-treated cybrids showed higher levels of IL-33 expression compared to the H-treated cybrids. Panel 3b: At 0-hours, the TGF-A expression levels were lower for the J-untreated cybrids (black solid line) compared to the H-untreated cybrids (grey solid line, p<0.0001). At 120 hours after UV-treatment, the TGF-A levels for both H-treated (grey broken line) and J-treated (black broken line) were higher than the untreated H (grey solid line) or J (black solid line) cybrids. Panel 3c: At 0-hours, the expression levels for EFEMP1 were lower in the J-untreated (black solid lines) compared to H-untreated cybrids (grey solid lines, p = 0.0001). By 120 hours, the J-treated cybrids (black broken line) and J-untreated cybrids (black solid line) showed greater fold increases of EFEMP1 expression compared to the H-treated (grey broken line) and H-untreated (grey solid line) cybrids. There were triplicate wells for each sample and the experiments were repeated twice. J cybrids, n = 3 different individuals; H cybrids, n = 3 different individuals.