Expression of pluripotency markers in chiPSc colonies.

Left column: staining of nuclei using DAPI (blue). Right column: the same colony stained with antibodies against several pluripotency markers including, Oct4, Sox2, Ssea1 and Nanog. Oct4- and Nanog-positive colonies are enlarged to show specificity of the staining in comparison to surrounding fibroblasts. 100x magnification was used for Oct4, Ssea1 and Nanog, 200x magnification for Sox2.