Expression of novel is-ncRNAs in human fetal tissues.

(A) Relative expression of 82 novel is-ncRNAs extracted from human fetal brain tissue as analysed by microarray. The leftmost column (shades of blue) shows the expression levels of different novel ncRNAs in fetal brain tissue relative to the average expression in this tissue. The five columns to the right (shades of green) show the expression of each novel ncRNA relative to its expression in fetal brain. (B) Relationship between conservation and expressional variation among fetal tissues. The figure shows PhastCons scores plotted against maximal expressional variation among fetal tissues of known (red) and novel (blue) is-ncRNAs. (C) Northern blot of the novel is-ncRNA nc013. Note the lack of expression in liver and thymus. (D) Northern blot analysis of 6 novel transcripts with predominant or unique expression in the human fetal brain. (E) Relative expression of 82 novel is-ncRNAs in human fetal brain during four gestation stages. The leftmost column (shades of blue) shows the expression levels of different novel ncRNAs in fetal brain tissue at 13 weeks of gestation. The four columns to the right (shades of green) show the expression of each novel ncRNA in fetal brain at gestation stages 14 weeks, 20 weeks and 24 weeks relative to its expression at 13 weeks of gestation. (F) A magnified view from Figure E, which represents the relative expression of 7 novel is-ncRNAs in human fetal brain during four gestation stages. (G) Expression of nine novel is-ncRNAs expression during fetal brain development, in three tumor cell lines and in one clinical brain tumor sample. snRNA U6 served as an internal loading control.

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