Expression of human NLGN1 and rat Nlgn1 cDNAs rescue the wild type phenotype for gentle touch response in nlg-1 defective mutants of C. elegans.

2012-06-18T00:45:30Z (GMT) by Fernando Calahorro Manuel Ruiz-Rubio

Data are quantified as percentage of positive response to gentle touch. Animals were touched ten times, alternating anterior and posterior part of the body. The measure was carried out by stroking an eyebrow hair across the body just behind the pharynx for the anterior touch response (A), or just before the anus for the posterior touch response (B). Rescues: Worm nlg-1 correspond to CRR104 transgenic strain (crrEx4 [pPD95.77 (Pnlg-1::nlg-1); pDD04NeoR (Pmyo-2::GFP)]); Human NLGN1 represent transgenic strains CRR103 (crrEx3 [pPD95.77 (Pnlg-1::NLGN1); pDD04NeoR (pmyo-2::GFP)]); Rat Nlgn1 correspond to transgenic strain CRR109 (crrEx9 [pPD95.77 (Pnlg-1::Nlgn1::EGFP); pBCN24NeoR]); Worm nlg-1 R437C is CRR105 strain (crrEx5 [pPD95.77 (Pnlg-1::nlg-1-R437C); pDD04NeoR (Pmyo-2::GFP)]); Human NLGN1 R453C is CRR107 strain (crrEx7 [pPD95.77 (Pnlg-1::NLGN1-R453C); pDD04NeoR (Pmyo-2::GFP)]); Human NLGN1 D396X is CRR108 (crrEx8 [pPD95.77 (Pnlg-1::NLGN1-D396X); pDD04NeoR (Pmyo-2::GFP)]). Control is CRR100 a transgenic strain containing the pDD04NeoR “empty vector". At least three different experiments were carried out with each strain (approximately 50 worms per experiment). Error bars indicate the SEM. The asterisk indicates significant differences (p≤0.001) calculated by ANOVA/Fisher's test.