Expression of genes associated with steroidogenesis, cell adhesion and proteolysis in the periovulatory follicle.
(A) Microarray fold expression changes at 1 and 22 h post peak LH surge in granulosa cells for few of the selected up and down regulated genes associated with specific biological process. (B) The genes selected by microarray analysis were also subjected to qPCR analysis and fold expression changes for granulosa cells and follicular wall collected at different time points are represented as bar graphs. Bar for each gene represents mean ± SEM fold expression change value at each time point (n = 3 animals). For each gene, bars with different alphabets above them are significantly different (p<0.05). More details on the analysis are provided in the results section.